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In a chronic wound situation the physiological balance between matrix metalloproteinases (MMPs) and growth factors is disturbed. This is associated with inadequate cell migration and proliferation as well as matrix degradation, which results in stalled healing.1,2 Geistlich Derma-Gide® supports the rebalancing of these factors.3In vitro studies led to the discovery of the following clinically relevant characteristics:
Matrix Metalloproteinases (MMP) Activity Geistlich Derma-Gide® is shown to modulate MMP activity.
Growth Factor Binding and Preservation The Geistlich Derma-Gide® matrix maintains growth factors in an active state in situ, which is necessary for wound healing.
Cell Migration Geistlich Derma-Gide® provides optimal structure for cell migration and attachment.
MMP-1, 2, and 9, which are most abundant in chronic wounds, were analyzed after 2h incubation with Geistlich Derma-Gide® in the supernatant. MMPs alone served as the control. Geistlich Derma-Gide® led to lower activity of MMP-1 (55% reduction, upper panel), MMP-2 (96% reduction, middle panel) and MMP-9 (66% reduction, lower panel) 2h after incubation compared to the control, therefore demonstrating modulation of MMP activity in this in vitro study.
Geistlich Derma-Gide® was incubated with healing-related growth factors or control solutions and extensively washed. After 0h or 72h the matrix was cultured with cells and the effect on cell activity was measured by gene expression. An equally strong activity was measured at both time points for TGF-β1, bFGF and VEGF, which are important for vascularization, reepithelialization, and tissue formation.
Human epidermal keratinocytes were seeded in Boyden chamber wells. In the lower compartment either basal medium (negative control), Geistlich Derma-Gide® or growth factor supplements (positive control) were added. After 12h the cell number in the lower compartment of each well was quantified. The well containing Geistlich Derma-Gide® was found to contain a number of cells equivalent to the positive control.
Keratinocytes, fibroblasts and endothelial cells were seeded and cultured on Geistlich Derma-Gide® and afterwards fixed and stained for imaging. Keratinocytes formed a stratified epidermis (upper right picture), fibroblasts an equally dispersed monolayer (green, lower right picture) and endothelial cells tube-like structures (red, lower left picture) on Geistlich Derma-Gide®.